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Volume 29, Number 7—July 2023
Dispatch

Cutaneous Pythiosis in 2 Dogs, Italy

Andrea PeanoComments to Author , Anna Rita Molinar Min, Alessandra Fondati, Erica Romano, Chiara Brachelente, Ilaria Porcellato, Andrea Amore, and Mario Pasquetti
Author affiliations: Università di Torino, Turin, Italy (A. Peano, A.R. Molinar Min, M. Pasquetti); Veterinaria Trastevere, Rome, Italy (A. Fondati); Ospedale Veterinario Gregorio VII, Rome (E. Romano); Università degli Studi di Perugia, Perugia, Italy (C. Brachelente, I. Porcellato); Ambulatorio Veterinario Cassia, Cesano, Italy (A. Amore)

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Figure 2

Histological analyses of cutaneous pythiosis in 2 dogs, Italy. A) Section from punch biopsy of cutaneous mass showing confluent pyogranulomatous and eosinophilic granulomas with central necrosis in the deep dermis and panniculus. Hematoxylin and eosin stain; original magnification ×100. B) Section from punch biopsy of cutaneous mass showing broad, irregular branching hyphae. Grocott methenamine silver stain; original magnification ×400. C) Culture (24-hour) of an aspirate from a mass found in case 2. Aspirate was cultured on Sabouraud dextrose agar without antimicrobial drugs, and showed submerged, colorless colonies with irregular radiate patterns. D) Microscopic appearance of the colonies indicating broad (4–10 µm in diameter), hyaline, and sparsely septate hyphae. Original magnification ×250.

Figure 2. Histological analyses of cutaneous pythiosis in 2 dogs, Italy. A) Section from punch biopsy of cutaneous mass showing confluent pyogranulomatous and eosinophilic granulomas with central necrosis in the deep dermis and panniculus. Hematoxylin and eosin stain; original magnification ×100. B) Section from punch biopsy of cutaneous mass showing broad, irregular branching hyphae. Grocott methenamine silver stain; original magnification ×400. C) Culture (24-hour) of an aspirate from a mass found in case 2. Aspirate was cultured on Sabouraud dextrose agar without antimicrobial drugs, and showed submerged, colorless colonies with irregular radiate patterns. D) Microscopic appearance of the colonies indicating broad (4–10 µm in diameter), hyaline, and sparsely septate hyphae. Original magnification ×250.

Main Article

Page created: May 17, 2023
Page updated: June 20, 2023
Page reviewed: June 20, 2023
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