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Volume 22, Number 4—April 2016
Letter

Follow-up of Ebola Patient, 2014–2015

Venkatesh Srinivas, Pragya D. Yadav, Veena Mittal, Gouri Chaubal, Mala Chhabra, Sanjay Mattoo, Arvind Rai, and Devendra T. MouryaComments to Author 
Author affiliations: National Centre for Disease Control, New Delhi, India (S. Venkatesh, V. Mittal, M. Chhabra, A. Rai); National Institute of Virology, Pune, India (P. Yadav, G. Chaubal, D.T. Mourya); Airport Authority of India, New Delhi (S. Matto)

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Table

Results of attempted real-time RT-PCR and virus isolation from semen of Ebola virus disease survivor, 2014–2015*

1
2
3
4
NCDC‡
 NIV§
 CPE
 Ct
 CPE
 Ct
 CPE
 Ct
 CPE
 Ct
1
 45
 17
 22.5
 Vero CCL81 None 28.5 None None None None None None
Vero E6
 None
 31.5
None
 None
None
 None
ND
 None
2
 64
 21
 24
 Vero CCL81 None None None None None None ND ND
Vero E6
 None
 32.5
None
 None
None
 None
ND
 ND
3¶
 77
 22
 ND
 NA
 NA
 NA
NA
 NA
NA
 NA
NA
 NA
4¶
 98
 26
 ND
 NA
 NA
 NA
NA
 NA
NA
 NA
NA
 NA
5¶
 111
 27
 ND
 NA
 NA
 NA
NA
 NA
NA
 NA
NA
 NA
6
 125
 30
 34
 Vero CCL81 None None None None ND ND ND ND
Vero E6
 None
 None
None
 None
ND
 ND
ND
 ND
7
 141
 28
 38
 Vero CCL81 None None None None ND ND ND ND
Vero E6
 None
 None
None
 None
ND
 ND
ND
 ND
8 165 28 35.0 Vero CCL81 None None None None ND ND ND ND
BHK-21 None None None None ND ND ND ND

*Ct, cycle threshold; CPE, cytopathic effect; NA, not applicable; NCDC, National Centre for Disease Control, New Delhi, India; ND, not done; NIV, National Institute of Virology, Pune, India; RT-PCR, reverse transcription PCR.
†Primers from http://www.crcf.sn/wp-content/uploads/2014/09/CDC-Ebola-International-Lab.pdf.
‡Primers from the Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
§Primers from (7).
¶Samples received at NCDC only.

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References
  1. World Health Organization. Eighth Meeting of the WHO Advisory Group on the EVD Response; 2015 Mar 20; Geneva. Geneva: The Organization; 2015 [cited 2015 Mar 25]. http://www.who.int/csr/disease/ebola/advisory-groups/8th-meeting-report.pdf?ua=1
  2. World Health Organization. WHO Statement on the Meeting of the International Health Regulations Emergency Committee Regarding the 2014 Ebola Outbreak in West Africa; 2014 Aug 8; Geneva. Geneva: The Organization; 2014 [cited 2014 Nov 3]. http://www.who.int/mediacentre/news/statements/2014/ebola-20140808/en/
  3. Osterholm  MT, Moore  KA, Kelley  NS, Brosseau  LM, Wong  G, Murphy  FA, Transmission of Ebola viruses: what we know and what we do not know. mBiol. 2015;6 [cited 2016 Jan 19]. http://dx.doi: DOIGoogle Scholar
  4. Bausch  DG, Towner  JS, Dowell  SF, Kaducu  F, Lukwiya  M, Sanchez  A, Assessment of the risk of Ebola virus transmission from bodily fluids and fomites. J Infect Dis. 2007;196(Suppl 2):S142–7. DOIPubMedGoogle Scholar
  5. Rodriguez  LL, De Roo  A, Guimard  Y, Trappier  SG, Sanchez  A, Bressler  D, Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995. J Infect Dis. 1999;179(Suppl 1):S170–6. DOIPubMedGoogle Scholar
  6. ProMEDmail. Ebola virus disease—ex Africa (39): India, worldwide susp. or quarantined. 2014 Nov 18 [cited 2015 Dec 23]. http://www.promedmail.org, archive no. 20141118.2966874.
  7. Towner  JS, Sealy  TK, Ksiazek  TG, Nichol  ST. High-throughput molecular detection of hemorrhagic fever virus threats with applications for outbreak settings. J Infect Dis. 2007;196(Suppl 2):S205–12. DOIPubMedGoogle Scholar
  8. Ksiazek  TG, Rollin  PE, Williams  AJ, Bressler  DS, Martin  ML, Swanepoel  M, Clinical virology of Ebola hemorrhagic fever (EHF): virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Democratic Republic of the Congo, 1995. J Infect Dis. 1999;179(Suppl 1):S177–87. DOIPubMedGoogle Scholar
  9. Drosten  C, Göttig  S, Schilling  S, Asper  M, Panning  M, Schmitz  H, Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol. 2002;40:2323–30. DOIPubMedGoogle Scholar
  10. O'Dempsey  T, Khan  SH, Bausch  DG. Rethinking the discharge policy for Ebola convalescents in an accelerating epidemic. Am J Trop Med Hyg. 2015;92:238–9. DOIPubMedGoogle Scholar
  11. Deen  GF, Knust  B, Broutet  N, Sesay  FR, Formenty  P. Ross  C Ebola RNA persistence in semen of Ebola virus disease survivors—preliminary report. N Engl J Med. 2015 Oct 14 [cited 2015 Oct 20]. DOIGoogle Scholar

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